ChromasPro 2.2.0

About ChromasPro

ChromasPro is suitable for DNA sequence assembly projects up to a few megabases, and basic sequence editing and analysis. It is able to assemble data from Sanger sequencers such as ABI, and 454 and Illumina next-generation sequencers, with up to 1,000,000 sequences if 8 Gb RAM is available. ChromasPro has the following features:

• Open sequences in Applied Biosystems .ab1, Staden Chromatogram (SCF and ZTR), 454 SFF, FASTA, FASTQ, EMBL, GenBank, SwissProt, GenPept, GCG RSF and plain text formats.
• Save sequences in .ab1, .scf, .fasta and .fastq formats.
• Assemble overlapping sequences using a graphical interface, generate a consensus and highlight ambiguities for editing.
• Use quality data to automatically remove low quality sequence for improved sequence assembly.
• Use a reference sequence as a scaffold for assembly.
• Generate restriction site and fragment maps, and list cutters, non-cutters and fragments.
• Map open reading frames, aided by a G+C frame plot, and translate ORFs with one click.
• Print chromatograms, restriction site and fragment maps, and open reading frame maps.
• Perform nucleotide and protein BLAST searches through the NCBI web site.
• Perform multiple alignments by interfacing with ClustalW.
• Reverse & complement sequences and chromatograms.
• Search for sequences by exact matching or optimal alignment.
• Display translations when editing nucleotide sequences.
• Perform reverse translations and plot nucleotide degeneracy.
• Plot hydrophilicity and antigenicity of proteins.
• Copy an image of a chromatogram section for pasting into documents or presentations.